For my project I am engaged in the investigation of different types of G-quadruplex DNA or RNA binding proteins which could be identified in a proteomic approach. For this I will perform overexpression and purification of candidate proteins and analyze their interaction with various oligonucleotide structures – not only with G-quadruplex, but also with triplex, double stranded and single stranded nucleic acids.
The project is carried out in cooperation with the Institut Curie in Paris.
I´m not in the lab anymore as I´m currently writing my thesis. My research is all about quantification of epigenetic markers along genomic DNA. My focus lies on simultaneous detection of 5mC and 5hmC using two-step DNA modification reactions. First, reactive groups are transferred sequence-specifically using methyltransferases and β-glycosyltransferases. In a second step, bioorthogonal click-chemistry is used to establish fluorescent barcodes on the DNA by fluroescence microscopy. With these barcodes it is possible to obtain quantitative information on the epigenetic state of the DNA substrate.
I´m not in the lab anymore as I´m currently writing my thesis. My research is all about DNA barcoding. I use DNA methyltransferases to specifically transfer reporter groups to DNA. The modified DNA molecules are analyzed using fluorescence microscopy and nanopores/nanochannels. Another aspect of my work includes the development of computational methods to quantify the efficiency of a certain DNA barcoding method.
My project is about engineering activity of DNA methyltransferases with various cofactor analogues for DNA labeling. In cooperation with the group of Giulia Rossetti at the Forschungszentrum Jülich, I am performing computational simulations to dock cofactor-methyltransferase interactions. Results from these in silico experiments are then used to optimize catalytic activities by organic synthesis of new cofactor analogues or site-directed mutagenesis of proteins.
Much of my work involves protein expression and purification and doing restriction modifications assays.